Western blotting analysis using RHOC antibody (Cat#65872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RHOC antibody (Cat#65872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using RHOC antibody (Cat#65872). RHOC expression in wild-type (WT) and RHOC shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RHOC antibody (Cat#65872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with RHOC antibody (Cat#65872, 1:1,000). Nuclei were stained blue with DAPI; RHOC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HT-1080 cells using RHOC antibody (Cat#65872, 1:1,000), Top panel: wild-type (WT); Bottom panal: RHOC shRNA knockdown (KD). Nuclei were stained blue with DAPI; RHOC was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of RHOC expression in C2C12 cells using RHOC antibody (Cat#65872, 1:2,000). Green, isotype control; red, RHOC.
