Western blotting analysis using hepatocyte nuclear factor 4 alpha antibody (Cat#2894). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of hepatocyte nuclear factor 4 alpha expression in HepG2 cells using hepatocyte nuclear factor 4 alpha antibody (Cat#2894, 1:2,000). Green, isotype control; red, Hepatocyte nuclear factor 4 alpha.
