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Home>Product>Primary Antibody

KD-Validated RHOC Mouse mAb

#65872

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shRNA Knockdown Validated
Western blotting analysis using RHOC antibody (Cat#65872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RHOC antibody (Cat#65872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using RHOC antibody (Cat#65872). RHOC expression in wild-type (WT) and RHOC shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RHOC antibody (Cat#65872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse small intestine tissue using RHOC antibody (Cat#65872, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of C2C12 cells with RHOC antibody (Cat#65872, 1:1,000). Nuclei were stained blue with DAPI; RHOC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HT-1080 cells using RHOC antibody (Cat#65872, 1:1,000), Top panel: wild-type (WT); Bottom panal: RHOC shRNA knockdown (KD). Nuclei were stained blue with DAPI; RHOC was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RHOC expression in C2C12 cells using RHOC antibody (Cat#65872, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RHOC.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RHOC
  • Catalog：
    65872
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated RHOC Mouse mAb
Aliases RHOC; Ras Homolog Family Member C; ARH9; ARHC; Rho-Related GTP-Binding Protein RhoC; Ras Homolog Gene Family, Member C; Rho CDNA Clone 9; H9; Epididymis Secretory Sperm Binding Protein; Small GTP Binding Protein RhoC; RAS-Related Homolog 9; Oncogene RHO H9; RhoC GTPase; RHOH9
Background

Gene Name: RHOC

NCBI Gene Entry: 389

UniProt Entry: P08134

Application Information

Molecular Weight: Predicted, 22 kDa; observed, 22 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5595

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human RHOC
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:250-1:500
Note This product is for research use only.
Data
  • Western blotting analysis using RHOC antibody (Cat#65872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RHOC antibody (Cat#65872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using RHOC antibody (Cat#65872). RHOC expression in wild-type (WT) and RHOC shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RHOC antibody (Cat#65872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded mouse small intestine tissue using RHOC antibody (Cat#65872, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of C2C12 cells with RHOC antibody (Cat#65872, 1:1,000). Nuclei were stained blue with DAPI; RHOC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Immunocytochemical staining of HT-1080 cells using RHOC antibody (Cat#65872, 1:1,000), Top panel: wild-type (WT); Bottom panal: RHOC shRNA knockdown (KD). Nuclei were stained blue with DAPI; RHOC was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of RHOC expression in C2C12 cells using RHOC antibody (Cat#65872, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RHOC.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RHOC
  • Catalog：
    65872
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using RHOC antibody (Cat#65872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RHOC antibody (Cat#65872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using RHOC antibody (Cat#65872). RHOC expression in wild-type (WT) and RHOC shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RHOC antibody (Cat#65872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse small intestine tissue using RHOC antibody (Cat#65872, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of C2C12 cells with RHOC antibody (Cat#65872, 1:1,000). Nuclei were stained blue with DAPI; RHOC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunocytochemical staining of HT-1080 cells using RHOC antibody (Cat#65872, 1:1,000), Top panel: wild-type (WT); Bottom panal: RHOC shRNA knockdown (KD). Nuclei were stained blue with DAPI; RHOC was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RHOC expression in C2C12 cells using RHOC antibody (Cat#65872, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RHOC.
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