Western blotting analysis using taxilin alpha antibody (Cat#5949). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with taxilin alpha antibody (Cat#5949, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human ovarian carcinoma using taxilin alpha antibody (Cat#5949, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen
Recombinant protein of human TXLNA
Isotype
Mouse IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note
This product is for research use only.
Data
Western blotting analysis using taxilin alpha antibody (Cat#5949). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with taxilin alpha antibody (Cat#5949, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human ovarian carcinoma using taxilin alpha antibody (Cat#5949, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using taxilin alpha antibody (Cat#5949). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with taxilin alpha antibody (Cat#5949, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human ovarian carcinoma using taxilin alpha antibody (Cat#5949, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000793s ]
SELECT * FROM `goods` WHERE `code` = '5949' LIMIT 1 [ RunTime:0.000525s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000472s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000327s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000380s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6909 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000341s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000448s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6909 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000284s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000385s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000272s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.073326s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6025,6485,7544,4986,10514,5782,9612,3854,5691,10416) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000827s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000464s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000220s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000489s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000226s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000309s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000396s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000224s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000255s ]
