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Home>Product>Primary Antibody

HSP90AB1 Mouse mAb

#6799

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Western blotting analysis using HSP90AB1 antibody (Cat#6799). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90AB1 antibody (Cat#6799, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with HSP90AB1 antibody (Cat#6799, 1:1,000). Nuclei were stained blue with DAPI; HSP90AB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HSP90AB1 expression in HepG2 cells using HSP90AB1 antibody (Cat#6799, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HSP90AB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSP90AB1
  • Catalog：
    6799
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name HSP90AB1 Mouse mAb
Aliases HSP90AB1; Heat Shock Protein 90 Alpha Family Class B Member 1; HSPC2; HSPCB; Heat Shock Protein 90kDa Alpha (Cytosolic), Class B Member 1; Heat Shock Protein Family C Member 3; Heat Shock 90kD Protein 1, Beta; Heat Shock Protein HSP 90-Beta; Heat Shock 84 KDa; HSP90B; HSP84; Heat Shock Protein 90kDa Alpha Family Class B Member 1; Heat Shock 90kDa Protein 1, Beta; Heat Shock Protein 90 KDa; HSP90-Beta; D6S182; HSP 90; HSP 84; HSPC3
Background

Gene Name: HSP90AB1

NCBI Gene Entry: 3326

UniProt Entry: P08238

Application Information

Molecular Weight: Predicted, 83 kDa; observed, 85 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB895

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human HSP90AB1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HSP90AB1 antibody (Cat#6799). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90AB1 antibody (Cat#6799, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with HSP90AB1 antibody (Cat#6799, 1:1,000). Nuclei were stained blue with DAPI; HSP90AB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of HSP90AB1 expression in HepG2 cells using HSP90AB1 antibody (Cat#6799, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HSP90AB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSP90AB1
  • Catalog：
    6799
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using HSP90AB1 antibody (Cat#6799). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90AB1 antibody (Cat#6799, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with HSP90AB1 antibody (Cat#6799, 1:1,000). Nuclei were stained blue with DAPI; HSP90AB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HSP90AB1 expression in HepG2 cells using HSP90AB1 antibody (Cat#6799, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, HSP90AB1.
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