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KD-Validated AQP2 Recombinant Rabbit mAb

#61816

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using AQP2 antibody (Cat#61816). AQP2 expression in wild-type (WT) and AQP2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AQP2 antibody (Cat#61816, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of AQP2 expression in HepG2 cells using AQP2 antibody (Cat#61816, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AQP2.
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    AQP2
  • Catalog:
    61816
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name KD-Validated AQP2 Recombinant Rabbit mAb
Aliases AQP2; Aquaporin 2; Water Channel Protein For Renal Collecting Duct; Collecting Duct Water Channel Protein; Aquaporin 2 (Collecting Duct); ADH Water Channel; Aquaporin-CD; Aquaporin-2; AQP-CD; WCH-CD; AQP-2; Water-Channel Aquaporin 2; NDI2
Background

Gene Name: AQP2

NCBI Gene Entry: 359

UniProt Entry: P41181

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3855

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human AQP2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using AQP2 antibody (Cat#61816). AQP2 expression in wild-type (WT) and AQP2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AQP2 antibody (Cat#61816, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of AQP2 expression in HepG2 cells using AQP2 antibody (Cat#61816, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AQP2.
Western blotting analysis using AQP2 antibody (Cat#61816). AQP2 expression in wild-type (WT) and AQP2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AQP2 antibody (Cat#61816, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of AQP2 expression in HepG2 cells using AQP2 antibody (Cat#61816, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AQP2.
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