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Home>Product>Primary Antibody

KD-Validated Smac/Diablo Recombinant Rabbit mAb

#61458

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using Smac/Diablo antibody (Cat#61458). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Smac/Diablo antibody (Cat#61458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Smac/Diablo antibody (Cat#61458). Smac/Diablo expression in wild-type (WT) and DIABLO knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Smac/Diablo antibody (Cat#61458, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using DIABLO antibody (Cat#61458, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse testis using DIABLO antibody (Cat#61458, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with Smac/Diablo antibody (Cat#61458, 1:1,000). Nuclei were stained blue with DAPI; Smac/Diablo was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Smac/Diabl expression in HepG2 cells using Smac/Diabl antibody (Cat#61458, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Smac/Diabl.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DIABLO
  • Catalog：
    61458
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Smac/Diablo Recombinant Rabbit mAb
Aliases DIABLO; Diablo IAP-Binding Mitochondrial Protein; SMAC; Second Mitochondria-Derived Activator Of Caspase; DFNA64; Direct IAP-Binding Protein With Low PI; Diablo Homolog, Mitochondrial; DIABLO-S; FLJ25049; FLJ10537; Diablo, IAP-Binding Mitochondrial Protein; Diablo-Like Protein; Smac
Background

Gene Name: DIABLO

NCBI Gene Entry: 56616

UniProt Entry: Q9NR28

Application Information

Molecular Weight: Predicted, 27 kDa; observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2575

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Smac/Diablo
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using Smac/Diablo antibody (Cat#61458). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Smac/Diablo antibody (Cat#61458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using Smac/Diablo antibody (Cat#61458). Smac/Diablo expression in wild-type (WT) and DIABLO knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Smac/Diablo antibody (Cat#61458, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using DIABLO antibody (Cat#61458, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse testis using DIABLO antibody (Cat#61458, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with Smac/Diablo antibody (Cat#61458, 1:1,000). Nuclei were stained blue with DAPI; Smac/Diablo was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of Smac/Diabl expression in HepG2 cells using Smac/Diabl antibody (Cat#61458, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Smac/Diabl.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DIABLO
  • Catalog：
    61458
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Smac/Diablo antibody (Cat#61458). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Smac/Diablo antibody (Cat#61458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Smac/Diablo antibody (Cat#61458). Smac/Diablo expression in wild-type (WT) and DIABLO knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Smac/Diablo antibody (Cat#61458, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using DIABLO antibody (Cat#61458, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse testis using DIABLO antibody (Cat#61458, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with Smac/Diablo antibody (Cat#61458, 1:1,000). Nuclei were stained blue with DAPI; Smac/Diablo was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Smac/Diabl expression in HepG2 cells using Smac/Diabl antibody (Cat#61458, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Smac/Diabl.
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