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Home>Product>Primary Antibody

Transglutaminase 2 Mouse mAb

#5382

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Western blotting analysis using transglutaminase 2 antibody (Cat#5382). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with transglutaminase 2 antibody (Cat#5382, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using transglutaminase 2 antibody (Cat#5382, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Transglutaminase 2 expression in HT-1080 cells using Transglutaminase 2 antibody (Cat#5382, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Transglutaminase 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TGM2
  • Catalog：
    5382
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name Transglutaminase 2 Mouse mAb
Aliases TGM2; Transglutaminase 2; TGC; C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase; Protein-Glutamine Noradrenalinyltransferase TGM2; Protein-Glutamine Gamma-Glutamyltransferase 2; Protein-Glutamine Histaminyltransferase TGM2; Protein-Glutamine Dopaminyltransferase TGM2; Protein-Glutamine Serotonyltransferase TGM2; Protein-Glutamine Deamidase TGM2; Erythrocyte Transglutaminase; Tissue Transglutaminase; Transglutaminase II; Protein G Alpha(H); Transglutaminase C; Transglutaminase H; Isopeptidase TGM2; Heart G Alpha(H); HhG Alpha(H); EC 2.3.2.13; TGase II; TGase C; TGase H; TGase-2; TTgase; TG(C); G(H); HTG2; TG2; TTG; Transglutaminase 2 (C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase); Transglutaminase-2; EC 3.5.1.44; EC 3.4.-.-; EC 2.3.1.-
Background

Gene Name: TGM2

NCBI Gene Entry: 7052

UniProt Entry: P21980

Application Information

Molecular Weight: Predicted, 77 kDa; observed, 74 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1240

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human Transglutaminase 2
Isotype Mouse IgG1
Storage Buffer Supplied in crude ascites with 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using transglutaminase 2 antibody (Cat#5382). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with transglutaminase 2 antibody (Cat#5382, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using transglutaminase 2 antibody (Cat#5382, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of Transglutaminase 2 expression in HT-1080 cells using Transglutaminase 2 antibody (Cat#5382, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Transglutaminase 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TGM2
  • Catalog：
    5382
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using transglutaminase 2 antibody (Cat#5382). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with transglutaminase 2 antibody (Cat#5382, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using transglutaminase 2 antibody (Cat#5382, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Transglutaminase 2 expression in HT-1080 cells using Transglutaminase 2 antibody (Cat#5382, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Transglutaminase 2.
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