Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

KD-Validated RPS6KA1 Mouse mAb

#66857

Click to copy product information

Return to List

shRNA Knockdown Validated
Western blotting analysis using RPS6KA1 antibody (Cat#66857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KA1 antibody (Cat#66857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using RPS6KA1 antibody (Cat#66857). RPS6KA1 expression in wild-type (WT) and RPS6KA1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RPS6KA1 antibody (Cat#66857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with RPS6KA1 antibody (Cat#66857, 1:1,000). Nuclei were stained blue with DAPI; RPS6KA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KA1
  • Catalog：
    66857
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated RPS6KA1 Mouse mAb
Aliases RPS6KA1; Ribosomal Protein S6 Kinase A1; RSK1; MAPKAPK1; HU-1; MAP Kinase-Activated Protein Kinase 1a; 90 KDa Ribosomal Protein S6 Kinase 1; Ribosomal Protein S6 Kinase Alpha-1; MAPK-Activated Protein Kinase 1a; Ribosomal S6 Kinase 1; MAPKAP Kinase 1a; EC 2.7.11.1; MAPKAPK-1a; MAPKAPK1A; P90-RSK 1; P90RSK1; P90Rsk; P90S6K; RSK-1; DJ590P13.1 (Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1); Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1; S6K-Alpha 1; S6K-Alpha-1; EC 2.7.11; P90RSK; RSK
Background

Gene Name: RPS6KA1

NCBI Gene Entry: 6195

UniProt Entry: Q15418

Application Information

Molecular Weight: Predicted, 83 kDa; observed, 73 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1045

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human p90RSK
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RPS6KA1 antibody (Cat#66857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KA1 antibody (Cat#66857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using RPS6KA1 antibody (Cat#66857). RPS6KA1 expression in wild-type (WT) and RPS6KA1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RPS6KA1 antibody (Cat#66857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with RPS6KA1 antibody (Cat#66857, 1:1,000). Nuclei were stained blue with DAPI; RPS6KA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KA1
  • Catalog：
    66857
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using RPS6KA1 antibody (Cat#66857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KA1 antibody (Cat#66857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using RPS6KA1 antibody (Cat#66857). RPS6KA1 expression in wild-type (WT) and RPS6KA1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RPS6KA1 antibody (Cat#66857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with RPS6KA1 antibody (Cat#66857, 1:1,000). Nuclei were stained blue with DAPI; RPS6KA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.