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Home>Product>Primary Antibody

RPS6KA3 Recombinant Rabbit mAb

#5499

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Recombinant Antibody
Western blotting analysis using RPS6KA3 antibody (Cat#5499). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KA3 antibody (Cat#5499, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RPS6KA3 antibody (Cat#5499, 1:1,000). Nuclei were stained blue with DAPI; RPS6KA3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RPS6KA3 expression in HepG2 cells using RPS6KA3 antibody (Cat#5499, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RPS6KA3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KA3
  • Catalog：
    5499
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name RPS6KA3 Recombinant Rabbit mAb
Aliases RPS6KA3; Ribosomal Protein S6 Kinase A3; RSK2; HU-3; MAP Kinase-Activated Protein Kinase 1b; Ribosomal Protein S6 Kinase Alpha-3; Insulin-Stimulated Protein Kinase 1; MAPK-Activated Protein Kinase 1b; Ribosomal S6 Kinase 2; MAPKAP Kinase 1b; S6K-Alpha-3; EC 2.7.11.1; MAPKAPK-1b; MAPKAPK1B; P90-RSK 3; Pp90RSK2; ISPK-1; MRX19; RSK-2; CLS; Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 3; Epididymis Secretory Sperm Binding Protein; 90 KDa Ribosomal Protein S6 Kinase 3; S6K-Alpha3; EC 2.7.11; P90-RSK2; P90RSK3; XLID19; ISPK1; RSK
Background

Gene Name: RPS6KA3

NCBI Gene Entry: 6197

UniProt Entry: P51812

Application Information

Molecular Weight: Predicted, 84 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1780

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human p90 S6K alpha 3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RPS6KA3 antibody (Cat#5499). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KA3 antibody (Cat#5499, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with RPS6KA3 antibody (Cat#5499, 1:1,000). Nuclei were stained blue with DAPI; RPS6KA3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of RPS6KA3 expression in HepG2 cells using RPS6KA3 antibody (Cat#5499, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RPS6KA3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KA3
  • Catalog：
    5499
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using RPS6KA3 antibody (Cat#5499). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RPS6KA3 antibody (Cat#5499, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RPS6KA3 antibody (Cat#5499, 1:1,000). Nuclei were stained blue with DAPI; RPS6KA3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RPS6KA3 expression in HepG2 cells using RPS6KA3 antibody (Cat#5499, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RPS6KA3.
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