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PTPA Mouse mAb

#6326

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Western blotting analysis using PTPA antibody (Cat#6326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTPA antibody (Cat#6326, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PTPA expression in HepG2 cells using PTPA antibody (Cat#6326, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PTPA.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PTPA
  • Catalog:
    6326
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name PTPA Mouse mAb
Aliases PTPA; Protein Phosphatase 2 Phosphatase Activator; Phosphotyrosyl Phosphatase Activator; PPP2R4; PR53; Serine/Threonine-Protein Phosphatase 2A Regulatory Subunit B'; Protein Phosphatase 2A Activator, Regulatory Subunit 4; Protein Phosphatase 2A, Regulatory Subunit B' (PR 53); Serine/Threonine-Protein Phosphatase 2A Activator; Protein Phosphatase 2 Regulatory Subunit 4; PP2A Phosphatase Activator; Serine/Threonine-Protein Phosphatase 2A Regulatory Subunit 4; Protein Phosphatase 2A Regulatory Subunit 4; PP2A, Subunit B', PR53 Isoform; EC 5.2.1.8; PARK25; PP2A
Background

Gene Name: PTPA

NCBI Gene Entry: 5524

UniProt Entry: Q15257

Application Information

Molecular Weight: Predicted, 41 kDa; observed, 37-41 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8590

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human PTPA
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PTPA antibody (Cat#6326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTPA antibody (Cat#6326, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PTPA expression in HepG2 cells using PTPA antibody (Cat#6326, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PTPA.
Western blotting analysis using PTPA antibody (Cat#6326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PTPA antibody (Cat#6326, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PTPA expression in HepG2 cells using PTPA antibody (Cat#6326, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PTPA.
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