Western blotting analysis using Flag tag antibody (Cat#T024). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with Flag tag antibody (Cat#T024, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
The FLAG tag is a short peptide sequence (DYKDDDDK) commonly used in molecular biology to facilitate the detection and purification of recombinant proteins. Its small size minimizes interference with protein function and can be specifically recognized by anti-FLAG tag antibodies. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
Application Information
Molecular Weight: Recombinant protein dependent
Clonality: Mouse monoclonal antibody
Clone ID: 25GB7030
Species Reactivity: Recombinant protein
Applications Tested: Western blotting (WB)
Immunogen
A synthesized peptide derived from DYKDDDDK
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Data
Western blotting analysis using Flag tag antibody (Cat#T024). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with Flag tag antibody (Cat#T024, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using Flag tag antibody (Cat#T024). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with Flag tag antibody (Cat#T024, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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