Western blotting analysis using CCAR2 antibody (Cat#5189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CCAR2 antibody (Cat#5189, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with CCAR2 antibody (Cat#5189, 1:1,000). Nuclei were stained blue with DAPI; CCAR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CCAR2 expression in HT-1080 cells using CCAR2 antibody (Cat#5189, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CCAR2.
CCAR2; Cell Cycle And Apoptosis Regulator 2; KIAA1967; DBC-1; NET35; DBC1; Cell Division Cycle And Apoptosis Regulator Protein; Cell Cycle And Apoptosis Regulator Protein; Deleted In Breast Cancer 1; P30 DBC; Deleted In Breast Cancer Gene 1 Protein; DBIRD Complex Subunit KIAA1967; P30DBC; DBC.1
Western blotting analysis using CCAR2 antibody (Cat#5189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CCAR2 antibody (Cat#5189, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with CCAR2 antibody (Cat#5189, 1:1,000). Nuclei were stained blue with DAPI; CCAR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CCAR2 expression in HT-1080 cells using CCAR2 antibody (Cat#5189, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CCAR2.
Western blotting analysis using CCAR2 antibody (Cat#5189). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CCAR2 antibody (Cat#5189, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with CCAR2 antibody (Cat#5189, 1:1,000). Nuclei were stained blue with DAPI; CCAR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CCAR2 expression in HT-1080 cells using CCAR2 antibody (Cat#5189, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CCAR2.
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