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Home>Product>Primary Antibody

DDX21 Recombinant Rabbit mAb

#4891

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Recombinant Antibody
Western blotting analysis using DDX21 antibody (Cat#4891). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDX21 antibody (Cat#4891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with DDX21 antibody (Cat#4891, 1:1,000). Nuclei were stained blue with DAPI; DDX21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DDX21 expression in HAP-1 cells using DDX21 antibody (Cat#4891, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DDX21.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DDX21
  • Catalog：
    4891
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name DDX21 Recombinant Rabbit mAb
Aliases DDX21; DExD-Box Helicase 21; Gu-Alpha; Nucleolar RNA Helicase 2; RH-II/GU; GURDB; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21; Nucleolar RNA Helicase Gu; Nucleolar RNA Helicase II; DEAD-Box Helicase 21; DEAD Box Protein 21; RH II/Gu; DEAD (Asp-Glu-Ala-Asp) Box Helicase 21; RNA Helicase II/Gu Alpha; EC 3.6.4.13; Gu Protein; RH-II/GuA; EC 3.6.1 47; II/Gu; GUA; RH
Background

Gene Name: DDX21

NCBI Gene Entry: 9188

UniProt Entry: Q9NR30

Application Information

Molecular Weight: Predicted, 87 kDa; observed, 87 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13615

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human DDX21
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using DDX21 antibody (Cat#4891). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDX21 antibody (Cat#4891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with DDX21 antibody (Cat#4891, 1:1,000). Nuclei were stained blue with DAPI; DDX21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of DDX21 expression in HAP-1 cells using DDX21 antibody (Cat#4891, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DDX21.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DDX21
  • Catalog：
    4891
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using DDX21 antibody (Cat#4891). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDX21 antibody (Cat#4891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with DDX21 antibody (Cat#4891, 1:1,000). Nuclei were stained blue with DAPI; DDX21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DDX21 expression in HAP-1 cells using DDX21 antibody (Cat#4891, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DDX21.
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