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Home>Product>Primary Antibody

WNK Lysine Deficient Protein Kinase 1 Recombinant Rabbit mAb

#2672

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Recombinant Antibody
Western blotting analysis using WNK1 antibody (Cat#2672). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WNK1 antibody (Cat#2672, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with WNK1 antibody (Cat#2672, 1:1,000). Nuclei were stained blue with DAPI; WNK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    WNK1
  • Catalog：
    2672
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name WNK Lysine Deficient Protein Kinase 1 Recombinant Rabbit mAb
Aliases WNK1; WNK Lysine Deficient Protein Kinase 1; PPP1R167; PRKWNK1; HSAN2; HSN2; Protein Phosphatase 1, Regulatory Subunit 167; Serine/Threonine-Protein Kinase WNK1; Protein Kinase With No Lysine 1; Erythrocyte 65 KDa Protein; EC 2.7.11.1; KDP; P65; Prostate-Derived Sterile 20-Like Kinase; Hereditary Sensory Neuropathy, Type II; Serine/Threonine-Protein Kinase WNK1; Serine/Threonine-Protein Kinase WNK1; Protein Kinase, Lysine Deficient 1; Protein Kinase Lysine-Deficient 1; Kinase Deficient Protein; EC 2.7.11; KIAA0344; HWNK1; PSK
Background

Gene Name: WNK1

NCBI Gene Entry: 65125

UniProt Entry: Q9H4A3

Application Information

Molecular Weight: Predicted, 251 kDa; observed, 280 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2490

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human WNK1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using WNK1 antibody (Cat#2672). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WNK1 antibody (Cat#2672, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with WNK1 antibody (Cat#2672, 1:1,000). Nuclei were stained blue with DAPI; WNK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    WNK1
  • Catalog：
    2672
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using WNK1 antibody (Cat#2672). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WNK1 antibody (Cat#2672, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with WNK1 antibody (Cat#2672, 1:1,000). Nuclei were stained blue with DAPI; WNK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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