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Home>Product>Primary Antibody

SMN1 Recombinant Rabbit mAb

#2673

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Recombinant Antibody
Western blotting analysis using SMN1 antibody (Cat#2673). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMN1 antibody (Cat#2673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9c2 cells with SMN1 antibody (Cat#2673, 1:1,000). Nuclei were stained blue with DAPI; SMN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMN1 expression in HAP-1 cells using SMN1 antibody (Cat#2673, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMN1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMN1
  • Catalog：
    2673
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name SMN1 Recombinant Rabbit mAb
Aliases SMN1; Survival Of Motor Neuron 1, Telomeric; SMNT; TDRD16A; Gemin-1; BCD541; GEMIN1; SMA1; SMA2; SMA3; Survival Motor Neuron Protein; Tudor Domain Containing 16A; Component Of Gems 1; SMA@; SMA; SMN; Spinal Muscular Atrophy (Werdnig-Hoffmann Disease, Kugelberg-Welander Disease); Survival Motor Neuron 1 Protein; T-BCD541; SMA4; SMNC
Background

Gene Name: SMN1

NCBI Gene Entry: 6606

UniProt Entry: Q16637

Application Information

Molecular Weight: Predicted, 32 kDa; observed, 36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2535

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human SMN1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SMN1 antibody (Cat#2673). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMN1 antibody (Cat#2673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of H9c2 cells with SMN1 antibody (Cat#2673, 1:1,000). Nuclei were stained blue with DAPI; SMN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SMN1 expression in HAP-1 cells using SMN1 antibody (Cat#2673, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMN1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMN1
  • Catalog：
    2673
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SMN1 antibody (Cat#2673). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMN1 antibody (Cat#2673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9c2 cells with SMN1 antibody (Cat#2673, 1:1,000). Nuclei were stained blue with DAPI; SMN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMN1 expression in HAP-1 cells using SMN1 antibody (Cat#2673, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMN1.
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