Western blotting analysis using LGI1 antibody (Cat#61141). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LGI1 antibody (Cat#61141, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using LGI1 antibody (Cat#61141). LGI1 expression in wild type (WT) and LGI1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LGI1 antibody (Cat#61141, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat# 201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of LGI1 expression in HepG2 cells using LGI1 antibody (Cat#61141, 1:2,000). Green, isotype control; red, LGI1.