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Home>Product>Primary Antibody

UBE2N Recombinant Rabbit mAb

#2738

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Recombinant Antibody
Western blotting analysis using ubiquitin conjugating enzyme E2 N antibody (Cat#2738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Ubiquitin conjugating enzyme E2 N antibody(Cat#2738, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitin conjugating enzyme E2 N was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Ubiquitin conjugating enzyme E2 N expression in HT-1080 cells using Ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Ubiquitin conjugating enzyme E2 N.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBE2N
  • Catalog：
    2738
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name UBE2N Recombinant Rabbit mAb
Aliases UBE2N; Ubiquitin Conjugating Enzyme E2 N; UbcH-Ben; UBC13; Ubiquitin-Conjugating Enzyme E2N (Homologous To Yeast UBC13); Ubiquitin-Conjugating Enzyme E2N (UBC13 Homolog, Yeast); Ubiquitin-Conjugating Enzyme E2 N; E2 Ubiquitin-Conjugating Enzyme N; Ubiquitin Carrier Protein N; Ubiquitin-Protein Ligase N; MGC8489; UbcH13; Bendless-Like Ubiquitin Conjugating Enzyme; Bendless-Like Ubiquitin-Conjugating Enzyme; Epididymis Secretory Protein Li 71; Ubiquitin-Conjugating Enzyme E2N; Ubiquitin Conjugating Enzyme E2N; Yeast UBC13 Homolog; UBCHBEN; UBC13; EC 2.3.2.23; EC 6.3.2.19; HEL-S-71; UBCHBEN; Ubc13; BLU
Background

Gene Name: UBE2N

NCBI Gene Entry: 7334

UniProt Entry: P61088

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2555

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Ube2N / Ubc13
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ubiquitin conjugating enzyme E2 N antibody (Cat#2738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with Ubiquitin conjugating enzyme E2 N antibody(Cat#2738, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitin conjugating enzyme E2 N was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Ubiquitin conjugating enzyme E2 N expression in HT-1080 cells using Ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Ubiquitin conjugating enzyme E2 N.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBE2N
  • Catalog：
    2738
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ubiquitin conjugating enzyme E2 N antibody (Cat#2738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Ubiquitin conjugating enzyme E2 N antibody(Cat#2738, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitin conjugating enzyme E2 N was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Ubiquitin conjugating enzyme E2 N expression in HT-1080 cells using Ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Ubiquitin conjugating enzyme E2 N.
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