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Solute Carrier Family 7 Member 11 Recombinant Rabbit mAb

#2775

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Recombinant Antibody
Western blotting analysis using solute carrier family 7 member 11 antibody (Cat#2775). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 7 member 11 antibody (Cat#2775, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Solute carrier family 7 member 11 antibody (Cat#2775, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 7 member 11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Solute carrier family 7 member 11 expression in HepG2 cells using Solute carrier family 7 member 11 antibody (Cat#2775, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 7 member 11.
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Unit price:
$69.00
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1
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  • Gene Symbol:
    SLC7A11
  • Catalog:
    2775
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name Solute Carrier Family 7 Member 11 Recombinant Rabbit mAb
Aliases SLC7A11; Solute Carrier Family 7 Member 11; XCT; Solute Carrier Family 7 (Anionic Amino Acid Transporter Light Chain, Xc- System), Member 11; Calcium Channel Blocker Resistance Protein CCBR1; Amino Acid Transport System Xc-; Cystine/Glutamate Transporter; Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11; CCBR1
Background

Gene Name: SLC7A11

NCBI Gene Entry: 23657

UniProt Entry: Q9UPY5

Application Information

Molecular Weight: Predicted, 55 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2215

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human xCT
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using solute carrier family 7 member 11 antibody (Cat#2775). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 7 member 11 antibody (Cat#2775, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Solute carrier family 7 member 11 antibody (Cat#2775, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 7 member 11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Solute carrier family 7 member 11 expression in HepG2 cells using Solute carrier family 7 member 11 antibody (Cat#2775, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 7 member 11.
Western blotting analysis using solute carrier family 7 member 11 antibody (Cat#2775). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 7 member 11 antibody (Cat#2775, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Solute carrier family 7 member 11 antibody (Cat#2775, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 7 member 11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Solute carrier family 7 member 11 expression in HepG2 cells using Solute carrier family 7 member 11 antibody (Cat#2775, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 7 member 11.
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