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Home>Product>Primary Antibody

HSPA5 Mouse mAb

#2840

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Western blotting analysis using GRP78 antibody (Cat#2840). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GRP78 antibody (Cat#2840, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with GRP78 antibody (Cat#2840, 1:1,000). Nuclei were stained blue with DAPI; GRP78 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPA5
  • Catalog：
    2840
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name HSPA5 Mouse mAb
Aliases HSPA5; Heat Shock Protein Family A (Hsp70) Member 5; BiP; GRP78; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Immunoglobulin Heavy Chain-Binding Protein; Heat Shock Protein 70 Family Protein 5; Heat Shock Protein Family A Member 5; Endoplasmic Reticulum Chaperone BiP; Glucose-Regulated Protein, 78kDa; 78 KDa Glucose-Regulated Protein; Binding-Immunoglobulin Protein; HSP70 Family Protein 5; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD); Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78; Epididymis Secretory Sperm Binding Protein Li 89n; EC 3.6.4.10; HEL-S-89n; GRP-78
Background

Gene Name: HSPA5

NCBI Gene Entry: 3309

UniProt Entry: P11021

Application Information

Molecular Weight: Predicted, 72 kDa; observed, 72 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB2790

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human GRP78
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using GRP78 antibody (Cat#2840). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GRP78 antibody (Cat#2840, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with GRP78 antibody (Cat#2840, 1:1,000). Nuclei were stained blue with DAPI; GRP78 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSPA5
  • Catalog：
    2840
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using GRP78 antibody (Cat#2840). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GRP78 antibody (Cat#2840, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with GRP78 antibody (Cat#2840, 1:1,000). Nuclei were stained blue with DAPI; GRP78 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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