KD-Validated HSPA12A Recombinant Rabbit mAb - Product

KD-Validated HSPA12A Recombinant Rabbit mAb

#61420

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Recombinant Antibody
Knockdown Validated

Western blotting analysis using HSPA12A antibody (Cat#61420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using HSPA12A antibody (Cat#61420). HSPA12A expression in wild-type (WT) and HSPA12A knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Immunohistochemistry was performed on paraffin-embedded mouse brain using HSPA12A antibody (Cat#61420, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

HSPA12A
Catalog：

61420
Application：

WB, IHC-P
Species Reactivity：

H, M

Information

Data

Information

Product Name	KD-Validated HSPA12A Recombinant Rabbit mAb
Aliases	HSPA12A; Heat Shock Protein Family A (Hsp70) Member 12A; KIAA0417; Heat Shock 70 KDa Protein 12A; Heat Shock 70kDa Protein 12A; Heat Shock 70kD Protein 12A; FLJ13874
Background	Gene Name: HSPA12A NCBI Gene Entry: 259217 UniProt Entry: O43301
Application Information	Molecular Weight: Predicted, 75 kDa; observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB2475 Species Reactivity: Human,mouse Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen	A synthesized peptide derived from human HSPA12A
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note	This product is for research use only.

Data

Western blotting analysis using HSPA12A antibody (Cat#61420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HSPA12A antibody (Cat#61420). HSPA12A expression in wild-type (WT) and HSPA12A knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse brain using HSPA12A antibody (Cat#61420, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

HSPA12A
Catalog：

61420
Application：

WB, IHC-P
Species Reactivity：

H, M

Datasheet

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