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Prominin Recombinant Rabbit mAb

#2889

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Recombinant Antibody
Western blotting analysis using CD133 antibody (Cat#2889). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD133 antibody (Cat#2889, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD133 expression in HAP-1 cells using CD133 antibody (Cat#2889, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD133.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PROM1
  • Catalog:
    2889
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name Prominin Recombinant Rabbit mAb
Aliases PROM1; Prominin ; CORD12; PROML1; AC133; CD133; RP41; Retinitis Pigmentosa 41, Cone-Rod Dystrophy 1; Prominin-Like Protein 1; AC133 Antigen; Antigen AC133; Prominin-1; MCDR2; STGD4; Stargardt Disease 4 (Autosomal Dominant); Hematopoietic Stem Cell Antigen; Macular Dystrophy, Retinal 2; Prominin (Mouse)-Like 1; CD133 Antigen; HProminin; MSTP061
Background

Gene Name: PROM1

NCBI Gene Entry: 8842

UniProt Entry: O43490

Application Information

Molecular Weight: Predicted, 97 kDa; observed, 133 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3020

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human CD133
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD133 antibody (Cat#2889). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD133 antibody (Cat#2889, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of CD133 expression in HAP-1 cells using CD133 antibody (Cat#2889, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD133.
Western blotting analysis using CD133 antibody (Cat#2889). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD133 antibody (Cat#2889, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD133 expression in HAP-1 cells using CD133 antibody (Cat#2889, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD133.
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