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Caveolin 1 Recombinant Rabbit mAb

#2901

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Recombinant Antibody
Western blotting analysis using Caveolin 1 antibody (Cat#2901). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Caveolin 1 antibody (Cat#2901, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using caveolin 1 antibody (Cat#2901, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CAV1
  • Catalog:
    2901
  • Application:
    WB, IHC-P
  • Species Reactivity:
    H
Information
Product Name Caveolin 1 Recombinant Rabbit mAb
Aliases CAV1; Caveolin 1; Caveolin 1, Caveolae Protein, 22kDa; Caveolin-1; CAV; Caveolin 1, Caveolae Protein, 22kD; Cell Growth-Inhibiting Protein 32; MSTP085; BSCL3; LCCNS; VIP21; CGL3; PPH3
Background

Gene Name: CAV1

NCBI Gene Entry: 857

UniProt Entry: Q03135

Application Information

Molecular Weight: Predicted, 20 kDa; observed, 21-24 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3080

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)

Immunogen A synthesized peptide derived from human Caveolin-1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using Caveolin 1 antibody (Cat#2901). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Caveolin 1 antibody (Cat#2901, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using caveolin 1 antibody (Cat#2901, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using Caveolin 1 antibody (Cat#2901). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Caveolin 1 antibody (Cat#2901, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using caveolin 1 antibody (Cat#2901, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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