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Home>Product>Primary Antibody

IKBKB Recombinant Rabbit mAb

#2936

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Recombinant Antibody
Western blotting analysis using IKBKB antibody (Cat#2936). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKBKB antibody (Cat#2936, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
Immunocytochemical staining of HepG2 cells with IKBKB antibody (Cat#2936, 1:1,000). Nuclei were stained blue with DAPI; IKBKB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of IKBKB expression in HepG2 cells using IKBKB antibody (Cat#2936, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IKBKB.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IKBKB
  • Catalog：
    2936
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name IKBKB Recombinant Rabbit mAb
Aliases IKBKB; Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Beta; IKK-Beta; NFKBIKB; IKK2; IKKB; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells, Kinase Beta; Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta; Nuclear Factor NF-Kappa-B Inhibitor Kinase Beta; Serine/Threonine Protein Kinase IKBKB; I-Kappa-B-Kinase Beta; I-Kappa-B Kinase 2; EC 2.7.11.10; IKK-2; Nuclear Factor Kappa B Kinase Subunit Beta; EC 2.7.11.1; EC 2.7.11; IMD15A; IMD15B; IMD15; IKK-B; IkBKB
Background

Gene Name: IKBKB

NCBI Gene Entry: 3551

UniProt Entry: O14920

Application Information

Molecular Weight: Predicted, 87 kDa; observed, 87 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3250

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human IKK beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using IKBKB antibody (Cat#2936). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKBKB antibody (Cat#2936, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
  • Immunocytochemical staining of HepG2 cells with IKBKB antibody (Cat#2936, 1:1,000). Nuclei were stained blue with DAPI; IKBKB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of IKBKB expression in HepG2 cells using IKBKB antibody (Cat#2936, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IKBKB.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    IKBKB
  • Catalog：
    2936
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using IKBKB antibody (Cat#2936). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKBKB antibody (Cat#2936, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226)
Immunocytochemical staining of HepG2 cells with IKBKB antibody (Cat#2936, 1:1,000). Nuclei were stained blue with DAPI; IKBKB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of IKBKB expression in HepG2 cells using IKBKB antibody (Cat#2936, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, IKBKB.
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