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NR1H3 Recombinant Rabbit mAb

#2968

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Recombinant Antibody
Western blotting analysis using NR1H3 antibody (Cat#2968). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR1H3 antibody (Cat#2968, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with NR1H3 antibody (Cat#2968, 1:1,000). Nuclei were stained blue with DAPI; NR1H3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NR1H3 expression in HepG2 cells using NR1H3 antibody (Cat#2968, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NR1H3.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    NR1H3
  • Catalog:
    2968
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name NR1H3 Recombinant Rabbit mAb
Aliases NR1H3; Nuclear Receptor Subfamily 1 Group H Member 3; LXR-A; RLD-1; LXRa; Oxysterols Receptor LXR-Alpha; Nuclear Receptor Subfamily 1, Group H, Member 3; Liver X Receptor-Alpha; Liver X Receptor Alpha; LXRA
Background

Gene Name: NR1H3

NCBI Gene Entry: 10062

UniProt Entry: Q13133

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 48 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3405

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human LXR alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NR1H3 antibody (Cat#2968). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR1H3 antibody (Cat#2968, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with NR1H3 antibody (Cat#2968, 1:1,000). Nuclei were stained blue with DAPI; NR1H3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of NR1H3 expression in HepG2 cells using NR1H3 antibody (Cat#2968, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NR1H3.
Western blotting analysis using NR1H3 antibody (Cat#2968). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR1H3 antibody (Cat#2968, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with NR1H3 antibody (Cat#2968, 1:1,000). Nuclei were stained blue with DAPI; NR1H3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of NR1H3 expression in HepG2 cells using NR1H3 antibody (Cat#2968, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NR1H3.
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