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Clusterin Recombinant Rabbit mAb

#2987

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Recombinant Antibody
Western blotting analysis using Clusterin antibody (Cat#2987). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Clusterin antibody (Cat#2987, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with clusterin antibody (Cat#2987, 1:1,000). Nuclei were stained blue with DAPI; Clusterin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CLU
  • Catalog:
    2987
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name Clusterin Recombinant Rabbit mAb
Aliases CLU; Clusterin; TRPM-2; SGP-2; KUB1; Testosterone-Repressed Prostate Message 2; Sulfated Glycoprotein 2; Apolipoprotein J; SP-40; CLU1; CLU2; APOJ; CLI; Complement-Associated Protein SP-40,40; Complement Cytolysis Inhibitor; Complement Lysis Inhibitor; Ku70-Binding Protein 1; NA1/NA2; Clusterin (Complement Lysis Inhibitor, SP-40,40, Sulfated Glycoprotein 2, Testosterone-Repressed Prostate Message 2, Apolipoprotein J); Epididymis Secretory Sperm Binding Protein; Aging-Associated Gene 4 Protein; Aging-Associated Protein 4; APO-J; TRPM2; Apo-J; AAG4; SGP2
Background

Gene Name: CLU

NCBI Gene Entry: 1191

UniProt Entry: P10909

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 32-42, 65 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3500

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human Clusterin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Clusterin antibody (Cat#2987). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Clusterin antibody (Cat#2987, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with clusterin antibody (Cat#2987, 1:1,000). Nuclei were stained blue with DAPI; Clusterin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using Clusterin antibody (Cat#2987). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Clusterin antibody (Cat#2987, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with clusterin antibody (Cat#2987, 1:1,000). Nuclei were stained blue with DAPI; Clusterin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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