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CDC34 Recombinant Rabbit mAb

#3262

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Recombinant Antibody
Western blotting analysis using CDC34 antibody (Cat#3262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC34 antibody (Cat#3262, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with CDC34 antibody (Cat#3262, 1:1,000). Nuclei were stained blue with DAPI;CDC34 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CDC34 expression in HAP-1 cells using CDC34 antibody (Cat#3262, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,CDC34.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    CDC34
  • Catalog:
    3262
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name CDC34 Recombinant Rabbit mAb
Aliases CDC34; Cell Division Cycle 34, Ubiqiutin Conjugating Enzyme; UBE2R1; E2-CDC34; UBC3; (E3-Independent) E2 Ubiquitin-Conjugating Enzyme R1; Ubiquitin-Conjugating Enzyme E2-CDC34; Ubiquitin-Conjugating Enzyme E2 R1; E2 Ubiquitin-Conjugating Enzyme R1; Ubiquitin-Protein Ligase R1; UBCH3; Ubiquitin-Conjugating Enzyme E2-32 KDA Complementing; Ubiquitin-Conjugating Enzyme E2-32 KDa Complementing; Ubiquitin Conjugating Enzyme E2 R1; Cell Division Cycle 34 Homolog; Ubiquitin Carrier Protein; EC 2.3.2.23; EC 2.3.2.24; EC 6.3.2.19
Background

Gene Name: CDC34

NCBI Gene Entry: 997

UniProt Entry: P49427

Application Information

Molecular Weight: Predicted, 27 kDa; observed, 32 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4765

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human Cdc34
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CDC34 antibody (Cat#3262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC34 antibody (Cat#3262, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HAP-1 cells with CDC34 antibody (Cat#3262, 1:1,000). Nuclei were stained blue with DAPI;CDC34 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CDC34 expression in HAP-1 cells using CDC34 antibody (Cat#3262, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,CDC34.
Western blotting analysis using CDC34 antibody (Cat#3262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC34 antibody (Cat#3262, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with CDC34 antibody (Cat#3262, 1:1,000). Nuclei were stained blue with DAPI;CDC34 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CDC34 expression in HAP-1 cells using CDC34 antibody (Cat#3262, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,CDC34.
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