Western blotting analysis using phospho-presenilin 1 (Ser310) antibody (Cat#63205). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-presenilin 1 (Ser310) antibody (Cat#63205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-presenilin 1 (Ser310) antibody (Cat#63205). Phospho-presenilin 1 (Ser310) expression in wild-type (WT) and PSEN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with phospho-presenilin 1 (Ser310) antibody (Cat#63205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PSEN1, presenilin 1.
Immunocytochemical staining of Hela cells with Phospho-Presenilin 1 (Ser310) antibody (Cat#63205, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Presenilin 1 (Ser310) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Phospho-Presenilin 1 (Ser310) expression in HeLa cells using Phospho-Presenilin 1 (Ser310) antibody (Cat#63205, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Presenilin 1 (Ser310).
Validation of Presenilin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-Presenilin 1 (Ser310) antibody (Cat#63205, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
