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Methionine Sulfoxide Reductase B3 Recombinant Rabbit mAb

#2997

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Recombinant Antibody
Western blotting analysis using Methionine sulfoxide reductase B3 antibody (Cat#2997). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Methionine sulfoxide reductase B3 antibody (Cat#2997, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Methionine sulfoxide reductase B3 antibody (Cat#2997, 1:1,000). Nuclei were stained blue with DAPI; Methionine sulfoxide reductase B3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    MSRB3
  • Catalog:
    2997
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name Methionine Sulfoxide Reductase B3 Recombinant Rabbit mAb
Aliases MSRB3; Methionine Sulfoxide Reductase B3; Methionine-R-Sulfoxide Reductase B3; DKFZp686C1178; EC 1.8.4.12; FLJ36866; DFNB74; Methionine-R-Sulfoxide Reductase B3, Mitochondrial; Deafness, Autosomal Recessive 74; EC 1.8.4.14; MsrB3
Background

Gene Name: MSRB3

NCBI Gene Entry: 253827

UniProt Entry: Q8IXL7

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3550

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human MSRB3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Methionine sulfoxide reductase B3 antibody (Cat#2997). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Methionine sulfoxide reductase B3 antibody (Cat#2997, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Methionine sulfoxide reductase B3 antibody (Cat#2997, 1:1,000). Nuclei were stained blue with DAPI; Methionine sulfoxide reductase B3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using Methionine sulfoxide reductase B3 antibody (Cat#2997). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Methionine sulfoxide reductase B3 antibody (Cat#2997, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Methionine sulfoxide reductase B3 antibody (Cat#2997, 1:1,000). Nuclei were stained blue with DAPI; Methionine sulfoxide reductase B3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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