KD-Validated BAG5 Mouse mAb - Product - Hefei GenuIN Biotech Co., Ltd.

KD-Validated BAG5 Mouse mAb

#61841

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shRNA Knockdown Validated

Western blotting analysis using BAG5 antibody (Cat#61841). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BAG5 antibody (Cat#61841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using BAG5 antibody (Cat#61841). BAG5 expression in wild type (WT) and BAG5 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BAG5 antibody (Cat#61841, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of BAG5 expression in C2C12 cells using BAG5 antibody (Cat#61841, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, BAG5.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

BAG5
Catalog：

61841
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated BAG5 Mouse mAb
Aliases	BAG5; BAG Cochaperone 5; BAG Family Molecular Chaperone Regulator 5; Bcl-2-Associated Athanogene 5; BCL2 Associated Athanogene 5; BAG-5; KIAA0873; CMD2F
Background	Gene Name: BAG5 NCBI Gene Entry: 9529 UniProt Entry: Q9UL15
Application Information	Molecular Weight: Predicted, 51 kDa; observed, 48 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB3980 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human BAG5
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:100-1:1,000
Note	This product is for research use only.

Data

Western blotting analysis using BAG5 antibody (Cat#61841). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BAG5 antibody (Cat#61841, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using BAG5 antibody (Cat#61841). BAG5 expression in wild type (WT) and BAG5 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BAG5 antibody (Cat#61841, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of BAG5 expression in C2C12 cells using BAG5 antibody (Cat#61841, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, BAG5.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

BAG5
Catalog：

61841
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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