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Home>Product>Primary Antibody

EIF4A3 Recombinant Rabbit mAb

#3009

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Recombinant Antibody
Western blotting analysis using Eukaryotic translation initiation factor 4A3 antibody (Cat#3009). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Eukaryotic translation initiation factor 4A3 expression in HepG2 cells using Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Eukaryotic translation initiation factor 4A3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EIF4A3
  • Catalog：
    3009
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name EIF4A3 Recombinant Rabbit mAb
Aliases EIF4A3; Eukaryotic Translation Initiation Factor 4A3; KIAA0111; EIF4AIII; DDX48; Fal1; Eukaryotic Initiation Factor 4A-Like NUK-34; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 48; Eukaryotic Initiation Factor 4A-III; ATP-Dependent RNA Helicase EIF4A-3; ATP-Dependent RNA Helicase DDX48; Nuclear Matrix Protein 265; DEAD Box Protein 48; EIF-4A-III; EIF4A-III; NMP 265; Eukaryotic Translation Initiation Factor 4A, Isoform 3; Eukaryotic Translation Initiation Factor 4A Isoform 3; EC 3.6.4.13; HNMP 265; EC 3.6.1; NMP265; MUK34; NUK34; RCPS
Background

Gene Name: EIF4A3

NCBI Gene Entry: 9775

UniProt Entry: P38919

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 47 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3610

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human eIF4A3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Eukaryotic translation initiation factor 4A3 antibody (Cat#3009). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Eukaryotic translation initiation factor 4A3 expression in HepG2 cells using Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Eukaryotic translation initiation factor 4A3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EIF4A3
  • Catalog：
    3009
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Eukaryotic translation initiation factor 4A3 antibody (Cat#3009). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Eukaryotic translation initiation factor 4A3 expression in HepG2 cells using Eukaryotic translation initiation factor 4A3 antibody (Cat#3009, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Eukaryotic translation initiation factor 4A3.
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