Western blotting analysis using MAP3K2 antibody (Cat#66874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP3K2 antibody (Cat#66874, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAP3K2 antibody (Cat#66874). MAP3K2 expression in wild type (WT) and MAP3K2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with MAP3K2 antibody (Cat#66874, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:2,000-1:10,000
Note
This product is for research use only.
Data
Western blotting analysis using MAP3K2 antibody (Cat#66874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP3K2 antibody (Cat#66874, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAP3K2 antibody (Cat#66874). MAP3K2 expression in wild type (WT) and MAP3K2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with MAP3K2 antibody (Cat#66874, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAP3K2 antibody (Cat#66874). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP3K2 antibody (Cat#66874, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAP3K2 antibody (Cat#66874). MAP3K2 expression in wild type (WT) and MAP3K2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with MAP3K2 antibody (Cat#66874, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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