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Home>Product>Primary Antibody

KD-Validated MKI67 Mouse mAb

#61827

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Knockdown Validated
Western blotting analysis using marker of proliferation Ki-67 antibody (Cat#61827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with marker of proliferation Ki-67 antibody (Cat#61827, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using marker of proliferation Ki-67 antibody (Cat#61827). Marker of proliferation Ki-67 expression in wild-type (WT) and marker of proliferation Ki-67 (MKI67) knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with marker of proliferation Ki-67 antibody (Cat#61827, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using marker of proliferation Ki-67 antibody (Cat#61827, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using marker of proliferation Ki-67 antibody (Cat#61827, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HAP-1 cells with Marker of proliferation Ki-67 antibody (Cat#61827, 1:1,000). Nuclei were stained blue with DAPI; Marker of proliferation Ki-67 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Marker of proliferation Ki-67 expression in HAP-1 cells using Marker of proliferation Ki-67 antibody (Cat#61827, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Marker of proliferation Ki-67.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MKI67
  • Catalog：
    61827
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated MKI67 Mouse mAb
Aliases MKI67; Marker Of Proliferation Ki-67; Antigen Identified By Monoclonal Antibody Ki-67; PPP1R105; MIB-1; Protein Phosphatase 1, Regulatory Subunit 105; Molecular Immunology Borstel Antibody 1; Proliferation Marker Protein Ki-67; Antigen Ki67; Ki-67; Proliferation-Related Ki-67 Antigen; Antigen KI-67; MIB-; KIA
Background

Gene Name: MKI67

NCBI Gene Entry: 4288

UniProt Entry: P46013

Application Information

Molecular Weight: Predicted, 359 kDa; observed, 359 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB3920

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human MKI67
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using marker of proliferation Ki-67 antibody (Cat#61827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with marker of proliferation Ki-67 antibody (Cat#61827, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using marker of proliferation Ki-67 antibody (Cat#61827). Marker of proliferation Ki-67 expression in wild-type (WT) and marker of proliferation Ki-67 (MKI67) knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with marker of proliferation Ki-67 antibody (Cat#61827, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using marker of proliferation Ki-67 antibody (Cat#61827, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded human tonsillitis using marker of proliferation Ki-67 antibody (Cat#61827, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HAP-1 cells with Marker of proliferation Ki-67 antibody (Cat#61827, 1:1,000). Nuclei were stained blue with DAPI; Marker of proliferation Ki-67 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Marker of proliferation Ki-67 expression in HAP-1 cells using Marker of proliferation Ki-67 antibody (Cat#61827, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Marker of proliferation Ki-67.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MKI67
  • Catalog：
    61827
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using marker of proliferation Ki-67 antibody (Cat#61827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with marker of proliferation Ki-67 antibody (Cat#61827, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using marker of proliferation Ki-67 antibody (Cat#61827). Marker of proliferation Ki-67 expression in wild-type (WT) and marker of proliferation Ki-67 (MKI67) knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with marker of proliferation Ki-67 antibody (Cat#61827, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using marker of proliferation Ki-67 antibody (Cat#61827, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using marker of proliferation Ki-67 antibody (Cat#61827, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HAP-1 cells with Marker of proliferation Ki-67 antibody (Cat#61827, 1:1,000). Nuclei were stained blue with DAPI; Marker of proliferation Ki-67 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Marker of proliferation Ki-67 expression in HAP-1 cells using Marker of proliferation Ki-67 antibody (Cat#61827, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Marker of proliferation Ki-67.
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