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RBBP7 Mouse mAb

#5798

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Western blotting analysis using RBBP7 antibody (Cat#5798). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RBBP7 antibody (Cat#5798, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RBBP7 antibody (Cat#5798, 1:1,000). Nuclei were stained blue with DAPI; RBBP7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RBBP7 expression in HepG2 cells using RBBP7 antibody (Cat#5798, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RBBP7.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    RBBP7
  • Catalog:
    5798
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name RBBP7 Mouse mAb
Aliases RBBP7; RB Binding Protein 7, Chromatin Remodeling Factor; Histone Acetyltransferase Type B Subunit 2; Retinoblastoma-Binding Protein P46; Retinoblastoma-Binding Protein 7; RbAp46; G1/S Transition Control Protein-Binding Protein RbAp46; Nucleosome-Remodeling Factor Subunit RBAP46; Retinoblastoma-Binding Protein RbAp46; Histone-Binding Protein RBBP7; RBBP-7; Retinoblastoma Binding Protein 7; RB Binding Protein 7; RBAP46
Background

Gene Name: RBBP7

NCBI Gene Entry: 5931

UniProt Entry: Q16576

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5445

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human RBBP7
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RBBP7 antibody (Cat#5798). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RBBP7 antibody (Cat#5798, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with RBBP7 antibody (Cat#5798, 1:1,000). Nuclei were stained blue with DAPI; RBBP7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of RBBP7 expression in HepG2 cells using RBBP7 antibody (Cat#5798, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RBBP7.
Western blotting analysis using RBBP7 antibody (Cat#5798). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RBBP7 antibody (Cat#5798, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RBBP7 antibody (Cat#5798, 1:1,000). Nuclei were stained blue with DAPI; RBBP7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RBBP7 expression in HepG2 cells using RBBP7 antibody (Cat#5798, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RBBP7.
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