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Carbonic Anhydrase 1 Recombinant Rabbit mAb

#3023

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Recombinant Antibody
Western blotting analysis using Carbonic Anhydrase 1 antibody (Cat#3023). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Carbonic Anhydrase 1 antibody (Cat#3023, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Carbonic anhydrase 1 expression in H9c2 cells using Carbonic anhydrase 1 antibody (Cat#3023, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Carbonic anhydrase 1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CA1
  • Catalog:
    3023
  • Application:
    WB, FCM
  • Species Reactivity:
    M, R
Information
Product Name Carbonic Anhydrase 1 Recombinant Rabbit mAb
Aliases CA1; Carbonic Anhydrase 1; Carbonic Anhydrase I; Car1; Carbonate Dehydratase I; Cyanamide Hydratase CA1; Carbonic Anhydrase B; EC 4.2.1.1; CA-I; CAB; Epididymis Secretory Sperm Binding Protein; Epididymis Secretory Protein Li 11; EC 4.2.1.69; HEL-S-11
Background

Gene Name: CA1

NCBI Gene Entry: 759

UniProt Entry: P00915

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 29 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3680

Species Reactivity: Mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Carbonic Anhydrase 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using Carbonic Anhydrase 1 antibody (Cat#3023). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Carbonic Anhydrase 1 antibody (Cat#3023, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Carbonic anhydrase 1 expression in H9c2 cells using Carbonic anhydrase 1 antibody (Cat#3023, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Carbonic anhydrase 1.
Western blotting analysis using Carbonic Anhydrase 1 antibody (Cat#3023). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Carbonic Anhydrase 1 antibody (Cat#3023, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Carbonic anhydrase 1 expression in H9c2 cells using Carbonic anhydrase 1 antibody (Cat#3023, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Carbonic anhydrase 1.
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