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Home>Product>Primary Antibody

BUB3 Mitotic Checkpoint Protein Recombinant Rabbit mAb

#3024

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Recombinant Antibody
Western blotting analysis using BUB3 antibody (Cat#3024). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BUB3 antibody (Cat#3024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with BUB3 antibody (Cat#3024, 1:1,000). Nuclei were stained blue with DAPI; BUB3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BUB3 expression in HepG2 cells using BUB3 antibody (Cat#3024, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BUB3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BUB3
  • Catalog：
    3024
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name BUB3 Mitotic Checkpoint Protein Recombinant Rabbit mAb
Aliases BUB3; BUB3 Mitotic Checkpoint Protein; BUB3L; Mitotic Checkpoint Protein BUB3; BUB3 (Budding Uninhibited By Benzimidazoles 3, Yeast) Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast); BUB3 Budding Uninhibited By Benzimidazoles 3 Homolog; Budding Uninhibited By Benzimidazoles 3 Homolog; Testicular Tissue Protein Li 27; Budding Uninhibited By Benomyl; Mitotic Checkpoint Component; HBUB3
Background

Gene Name: BUB3

NCBI Gene Entry: 9184

UniProt Entry: O43684

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3685

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Bub3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using BUB3 antibody (Cat#3024). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BUB3 antibody (Cat#3024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with BUB3 antibody (Cat#3024, 1:1,000). Nuclei were stained blue with DAPI; BUB3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of BUB3 expression in HepG2 cells using BUB3 antibody (Cat#3024, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BUB3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BUB3
  • Catalog：
    3024
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using BUB3 antibody (Cat#3024). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BUB3 antibody (Cat#3024, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with BUB3 antibody (Cat#3024, 1:1,000). Nuclei were stained blue with DAPI; BUB3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of BUB3 expression in HepG2 cells using BUB3 antibody (Cat#3024, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, BUB3.
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