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Secretoglobin Family 1A Member 1 Recombinant Rabbit mAb

#3114

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Recombinant Antibody
Western blotting analysis using secretoglobin family 1A member 1 antibody (Cat#3114). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with secretoglobin family 1A member 1 antibody (Cat#3114, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with secretoglobin family 1A member 1 antibody (Cat#3114, 1:1,000). Nuclei were stained blue with DAPI; Secretoglobin family 1A member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of secretoglobin family 1A member 1 expression in HepG2 cells using secretoglobin family 1A member 1 antibody (Cat#3114, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, secretoglobin family 1A member 1.
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Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    SCGB1A1
  • Catalog:
    3114
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name Secretoglobin Family 1A Member 1 Recombinant Rabbit mAb
Aliases SCGB1A1; Secretoglobin Family 1A Member 1; CC10; CCSP; Uteroglobin; CC16; UGB; Club Cell Phospholipid-Binding Protein; Club Cells 10 KDa Secretory Protein; Club Secretory Protein 16; Club Cell Protein 16; Urinary Protein 1; Urine Protein 1; CCPBP; UP-1; UP1; Secretoglobin, Family 1A, Member 1 (Uteroglobin); Uteroglobin (Clara Cell Specific 10-KD Protein); Uteroglobin (Club-Cell Specific 10-KD Protein); Club Cell 10 KDa Secretory Protein; Blastokinin
Background

Gene Name: SCGB1A1

NCBI Gene Entry: 7356

UniProt Entry: P11684

Application Information

Molecular Weight: Predicted, 10 kDa; observed, 16 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4135

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human Uteroglobin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using secretoglobin family 1A member 1 antibody (Cat#3114). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with secretoglobin family 1A member 1 antibody (Cat#3114, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with secretoglobin family 1A member 1 antibody (Cat#3114, 1:1,000). Nuclei were stained blue with DAPI; Secretoglobin family 1A member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of secretoglobin family 1A member 1 expression in HepG2 cells using secretoglobin family 1A member 1 antibody (Cat#3114, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, secretoglobin family 1A member 1.
Western blotting analysis using secretoglobin family 1A member 1 antibody (Cat#3114). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with secretoglobin family 1A member 1 antibody (Cat#3114, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with secretoglobin family 1A member 1 antibody (Cat#3114, 1:1,000). Nuclei were stained blue with DAPI; Secretoglobin family 1A member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of secretoglobin family 1A member 1 expression in HepG2 cells using secretoglobin family 1A member 1 antibody (Cat#3114, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, secretoglobin family 1A member 1.
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