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KO-Validated KRT8 Mouse mAb

#71297

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Knockout Validated
Western blotting analysis using KRT8 antibody (Cat#71297). KRT8 expression in wild-type (WT) and KRT8 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KRT8 antibody (Cat#71297, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol:
    KRT8
  • Catalog:
    71297
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KO-Validated KRT8 Mouse mAb
Aliases KRT8; Keratin 8; CK-8; CYK8; K8; CARD2; K2C8; KO; Keratin, Type II Cytoskeletal 8; Type-II Keratin Kb8; Keratin 8, Type II; Keratin-8
Background

Gene Name: KRT8

NCBI Gene Entry: 3856

UniProt Entry: P05787

Application Information

Molecular Weight: Predicted, 54 kDa; observed, 48 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1580

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human KRT8
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using KRT8 antibody (Cat#71297). KRT8 expression in wild-type (WT) and KRT8 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KRT8 antibody (Cat#71297, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using KRT8 antibody (Cat#71297). KRT8 expression in wild-type (WT) and KRT8 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KRT8 antibody (Cat#71297, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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