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TRIM23 Recombinant Rabbit mAb

#5618

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Recombinant Antibody
Western blotting analysis using TRIM23 antibody (Cat#5618). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM23 antibody (Cat#5618, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TRIM23 expression in HepG2 cells using TRIM23 antibody (Cat#5618, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TRIM23.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    TRIM23
  • Catalog:
    5618
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name TRIM23 Recombinant Rabbit mAb
Aliases TRIM23; Tripartite Motif Containing 23; RNF46; ARD1; ARFD1; ADP-Ribosylation Factor Domain-Containing Protein 1; ADP-Ribosylation Factor Domain Protein 1, 64kDa; RING-Type E3 Ubiquitin Transferase TRIM23; Tripartite Motif-Containing Protein 23; E3 Ubiquitin-Protein Ligase TRIM23; GTP-Binding Protein ARD-1; RING Finger Protein 46; Tripartite Motif Protein TRIM23; Tripartite Motif-Containing 23; ARF Domain Protein 1; EC 2.3.2.27
Background

Gene Name: TRIM23

NCBI Gene Entry: 373

UniProt Entry: P36406

Application Information

Molecular Weight: Predicted, 64 kDa; observed, 58 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4545

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human TRIM23
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using TRIM23 antibody (Cat#5618). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM23 antibody (Cat#5618, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of TRIM23 expression in HepG2 cells using TRIM23 antibody (Cat#5618, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TRIM23.
Western blotting analysis using TRIM23 antibody (Cat#5618). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM23 antibody (Cat#5618, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TRIM23 expression in HepG2 cells using TRIM23 antibody (Cat#5618, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TRIM23.
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