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Home>Product>Primary Antibody

KD-Validated RPS6KB1 Rabbit mAb

#R0013

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Knockdown Validated
Western blotting analysis using anti-RPS6KB1 antibody (Cat#R0013). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti- RPS6KB1 antibody (Cat#R0013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RPS6KB1 antibody (Cat#R0013). RPS6KB1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RPS6KB1 antibody (Cat#R0013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-RPS6KB1 antibody (Cat#R0013, 1:1,000). Nuclei were stained blue with DAPI; RPS6KB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Flow cytometric analysis of RPS6KB1 expression in HepG2 cells using anti-RPS6KB1 antibody (Cat#R0013, 1:2,000). Green, isotype control; red, RPS6KB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KB1
  • Catalog：
    R0013
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KD-Validated RPS6KB1 Rabbit mAb
Aliases RPS6KB1; Ribosomal Protein S6 Kinase B1; S6K1; P70(S6K)-Alpha; STK14A; PS6K; S6K; Serine/Threonine-Protein Kinase 14A; Ribosomal Protein S6 Kinase Beta-1; Ribosomal Protein S6 Kinase I; EC 2.7.11.1; S6K-Beta-1; P70 S6KA; Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1; 70 KDa Ribosomal Protein S6 Kinase 1; Serine/Threonine Kinase 14 Alpha; P70 Ribosomal S6 Kinase Alpha; P70 S6 Kinase Alpha; P70 S6K-Alpha; P70-Alpha; P70-S6K 1; EC 2.7.11; P70-S6K; P70S6K1
Background

Gene Name: RPS6KB1

NCBI Gene Entry: 6198

UniProt Entry: P23443

Application Information

Molecular Weight: Predicted, 59 kDa; observed, 74 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: S-1278-2

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human RPS6KB1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using anti-RPS6KB1 antibody (Cat#R0013). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti- RPS6KB1 antibody (Cat#R0013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-RPS6KB1 antibody (Cat#R0013). RPS6KB1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RPS6KB1 antibody (Cat#R0013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with anti-RPS6KB1 antibody (Cat#R0013, 1:1,000). Nuclei were stained blue with DAPI; RPS6KB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Flow cytometric analysis of RPS6KB1 expression in HepG2 cells using anti-RPS6KB1 antibody (Cat#R0013, 1:2,000). Green, isotype control; red, RPS6KB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KB1
  • Catalog：
    R0013
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using anti-RPS6KB1 antibody (Cat#R0013). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti- RPS6KB1 antibody (Cat#R0013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RPS6KB1 antibody (Cat#R0013). RPS6KB1 expression in wild-type (WT) and RPS6KB1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RPS6KB1 antibody (Cat#R0013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with anti-RPS6KB1 antibody (Cat#R0013, 1:1,000). Nuclei were stained blue with DAPI; RPS6KB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Flow cytometric analysis of RPS6KB1 expression in HepG2 cells using anti-RPS6KB1 antibody (Cat#R0013, 1:2,000). Green, isotype control; red, RPS6KB1.
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