Cart

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News

Sign In/Register

Cart
  • Primary Antibody
  • Secondary Antibodies
  • Lentiviral Particles
  • Stable Cell Lines
  • Lysates
  • Reagents
  • Other Products
Home>Product>Primary Antibody

Phospho-GSK3 (Alpha + Beta)(Y216 + Y279) Recombinant Rabbit mAb

#4269

Click to copy product information

Return to List

Recombinant Antibody
Western blotting analysis using phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269, 1:1,000). Nuclei were stained blue with DAPI; Phospho-GSK3 (alpha + beta)(Y216 + Y279) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-GSK3 (alpha + beta)(Y216 + Y279) expression in C2C12 cells using Phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-GSK3 (alpha + beta)(Y216 + Y279).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GSK3A/GSK3B
  • Catalog：
    4269
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-GSK3 (Alpha + Beta)(Y216 + Y279) Recombinant Rabbit mAb
Aliases GSK3A; Glycogen Synthase Kinase 3 Alpha; Serine/Threonine-Protein Kinase GSK3A; Glycogen Synthase Kinase-3 Alpha; EC 2.7.11.26; GSK-3 Alpha; EC 2.7.11.1; EC 2.7.11; GSK3B; Glycogen Synthase Kinase 3 Beta; Serine/Threonine-Protein Kinase GSK3B; Glycogen Synthase Kinase-3 Beta; EC 2.7.11.26; GSK-3 Beta; EC 2.7.11.1; EC 2.7.11
Background

Gene Name: GSK3A/GSK3B

NCBI Gene Entry: 2931 , 2932

UniProt Entry: P49840 , P49841

Application Information

Molecular Weight: Predicted, 51 kDa; observed, 46,51 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10375

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-GSK3 alpha (Y279)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269, 1:1,000). Nuclei were stained blue with DAPI; Phospho-GSK3 (alpha + beta)(Y216 + Y279) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-GSK3 (alpha + beta)(Y216 + Y279) expression in C2C12 cells using Phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-GSK3 (alpha + beta)(Y216 + Y279).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GSK3A/GSK3B
  • Catalog：
    4269
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269, 1:1,000). Nuclei were stained blue with DAPI; Phospho-GSK3 (alpha + beta)(Y216 + Y279) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-GSK3 (alpha + beta)(Y216 + Y279) expression in C2C12 cells using Phospho-GSK3 (alpha + beta)(Y216 + Y279) antibody (Cat#4269, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-GSK3 (alpha + beta)(Y216 + Y279).
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$
View cart Empty cart
  • Phone Number

    +1 (301) 545-2395

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.