Western blotting analysis using DOP1B antibody (Cat#6407). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DOP1B antibody (Cat#6407, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with DOP1B antibody (Cat#6407, 1:1,000). Nuclei were stained blue with DAPI; DOP1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DOP1B expression in HAP-1 cells using DOP1B antibody (Cat#6407, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DOP1B.
DOP1B; DOP1 Leucine Zipper Like Protein B; KIAA0933; C21orf5; DOPEY2; Dopey Family Member 2; Protein DOP1B; Chromosome 21 Open Reading Frame 5; Homolog Of Yeast DOP1; Protein Dopey-2; 21orf5
Western blotting analysis using DOP1B antibody (Cat#6407). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DOP1B antibody (Cat#6407, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with DOP1B antibody (Cat#6407, 1:1,000). Nuclei were stained blue with DAPI; DOP1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DOP1B expression in HAP-1 cells using DOP1B antibody (Cat#6407, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DOP1B.
Western blotting analysis using DOP1B antibody (Cat#6407). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DOP1B antibody (Cat#6407, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with DOP1B antibody (Cat#6407, 1:1,000). Nuclei were stained blue with DAPI; DOP1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DOP1B expression in HAP-1 cells using DOP1B antibody (Cat#6407, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DOP1B.
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