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Home>Product>Primary Antibody

SART1 Recombinant Rabbit mAb

#3129

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Recombinant Antibody
Western blotting analysis using SART1 antibody (Cat#3129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SART1 antibody (Cat#3129, 1:50,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with SART1 antibody (Cat#3129, 1:1,000). Nuclei were stained blue with DAPI; SART1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SART1 expression in HepG2 cells using SART1 antibody (Cat#3129, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SART1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SART1
  • Catalog：
    3129
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name SART1 Recombinant Rabbit mAb
Aliases SART1; Spliceosome Associated Factor 1, Recruiter Of U4/U6.U5 Tri-SnRNP; Squamous Cell Carcinoma Antigen Recognized By T-Cells 1; SNRNP110; Snu66; Ara1; HAF; Squamous Cell Carcinoma Antigen Recognised By T Cells; Small Nuclear Ribonucleoprotein 110kDa (U4/U6.U5); SART1, U4/U6.U5 Tri-SnRNP-Associated Protein 1; U4/U6.U5 Tri-SnRNP-Associated 110 KDa Protein; U4/U6.U5 Tri-SnRNP-Associated Protein 1; Hypoxia Associated Factor; SNU66 Homolog; HSART-1; SART-1; HSnu66; Squamous Cell Carcinoma Antigen Recognized By T Cells 1; SART1(259) Protein;
SART1(800) Protein; Allergen Hom S 1; IgE Autoantigen; SART1259; HOMS1
Background

Gene Name: SART1

NCBI Gene Entry: 9092

UniProt Entry: O43290

Application Information

Molecular Weight: Predicted, 90 kDa; observed, 110 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4210

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human SNUT1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:10,000-1:50,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SART1 antibody (Cat#3129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SART1 antibody (Cat#3129, 1:50,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with SART1 antibody (Cat#3129, 1:1,000). Nuclei were stained blue with DAPI; SART1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SART1 expression in HepG2 cells using SART1 antibody (Cat#3129, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SART1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SART1
  • Catalog：
    3129
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SART1 antibody (Cat#3129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SART1 antibody (Cat#3129, 1:50,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with SART1 antibody (Cat#3129, 1:1,000). Nuclei were stained blue with DAPI; SART1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SART1 expression in HepG2 cells using SART1 antibody (Cat#3129, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SART1.
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