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KHDRBS1 Recombinant Rabbit mAb

#3135

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Recombinant Antibody
Western blotting analysis using KHDRBS1 antibody (Cat#3135). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KHDRBS1 antibody (Cat#3135, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with KHDRBS1 antibody (Cat#3135, 1:1,000). Nuclei were stained blue with DAPI; KHDRBS1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of KHDRBS1 expression in HeLa cells using KHDRBS1 antibody (Cat#3135, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, KHDRBS1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    KHDRBS1
  • Catalog:
    3135
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name KHDRBS1 Recombinant Rabbit mAb
Aliases KHDRBS1; KH RNA Binding Domain Containing, Signal Transduction Associated 1; Sam68; Src-Associated In Mitosis 68 KDa Protein; P62; KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 1; P21 Ras GTPase-Activating Protein-Associated P62; GAP-Associated Tyrosine Phosphoprotein P62; FLJ34027; P68; KH Domain Containing, RNA Binding, Signal Transduction Associated 1; GAP-Associated Tyrosine Phosphoprotein P62 (Sam68); SAM68
Background

Gene Name: KHDRBS1

NCBI Gene Entry: 10657

UniProt Entry: Q07666

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4235

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human SAM68
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using KHDRBS1 antibody (Cat#3135). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KHDRBS1 antibody (Cat#3135, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells with KHDRBS1 antibody (Cat#3135, 1:1,000). Nuclei were stained blue with DAPI; KHDRBS1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of KHDRBS1 expression in HeLa cells using KHDRBS1 antibody (Cat#3135, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, KHDRBS1.
Western blotting analysis using KHDRBS1 antibody (Cat#3135). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KHDRBS1 antibody (Cat#3135, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with KHDRBS1 antibody (Cat#3135, 1:1,000). Nuclei were stained blue with DAPI; KHDRBS1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of KHDRBS1 expression in HeLa cells using KHDRBS1 antibody (Cat#3135, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, KHDRBS1.
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