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Home>Product>Primary Antibody

CD84 Molecule Recombinant Rabbit mAb

#3140

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Recombinant Antibody
Western blotting analysis using CD84 antibody (Cat#3140). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD84 antibody (Cat#3140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with CD84 antibody (Cat#3140, 1:1,000). Nuclei were stained blue with DAPI; CD84 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD84 expression in HT-1080 cells using CD84 antibody (Cat#3140, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD84.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CD84
  • Catalog：
    3140
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name CD84 Molecule Recombinant Rabbit mAb
Aliases CD84; CD84 Molecule; SLAMF5; HCD84; MCD84; Signaling Lymphocytic Activation Molecule 5; Leukocyte Differentiation Antigen CD84; CD84 Antigen (Leukocyte Antigen); Cell Surface Antigen MAX.3; SLAM Family Member 5; Hly9-Beta; Leucocyte Differentiation Antigen CD84; Leukocyte Antigen CD84; CD84 Molecule; CD84 Antigen; LY9B
Background

Gene Name: CD84

NCBI Gene Entry: 8832

UniProt Entry: Q9UIB8

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 60-85 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5115

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CD84
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD84 antibody (Cat#3140). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD84 antibody (Cat#3140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with CD84 antibody (Cat#3140, 1:1,000). Nuclei were stained blue with DAPI; CD84 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CD84 expression in HT-1080 cells using CD84 antibody (Cat#3140, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD84.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CD84
  • Catalog：
    3140
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using CD84 antibody (Cat#3140). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD84 antibody (Cat#3140, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with CD84 antibody (Cat#3140, 1:1,000). Nuclei were stained blue with DAPI; CD84 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD84 expression in HT-1080 cells using CD84 antibody (Cat#3140, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CD84.
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