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CEACAM1 Recombinant Rabbit mAb

#5440

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Recombinant Antibody
Western blotting analysis using CEACAM1 antibody (Cat#5440). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CEACAM1 antibody (Cat#5440, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CEACAM1 expression in A549 cells using CEACAM1 antibody (Cat#5440, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CEACAM1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CEACAM1
  • Catalog:
    5440
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name CEACAM1 Recombinant Rabbit mAb
Aliases CEACAM1; CEA Cell Adhesion Molecule 1; BGP1; BGP; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein); Carcinoembryonic Antigen Related Cell Adhesion Molecule 1; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1; Cell Adhesion Molecule CEACAM1; CD66a Antigen; CD66a; Biliary Glycoprotein 1; Antigen CD66; BGP-1; BGPI
Background

Gene Name: CEACAM1

NCBI Gene Entry: 634

UniProt Entry: P13688

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 100-200 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1565

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human CEACAM1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using CEACAM1 antibody (Cat#5440). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CEACAM1 antibody (Cat#5440, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of CEACAM1 expression in A549 cells using CEACAM1 antibody (Cat#5440, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CEACAM1.
Western blotting analysis using CEACAM1 antibody (Cat#5440). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CEACAM1 antibody (Cat#5440, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CEACAM1 expression in A549 cells using CEACAM1 antibody (Cat#5440, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CEACAM1.
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