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Home>Product>Primary Antibody

KD-Validated Histone H3 (acetyl K27) Rabbit pAb

#R0015

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Knockdown Validated
Western blotting analysis using anti-histone H3 (acetyl K27) antibody (Cat#R0015). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-histone H3 (acetyl K27) antibody (Cat#R0015). Histone H3 (acetyl K27) expression in wild-type (WT) and H3C1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of H3C1 expression in HT-1080 cells using anti-H3C1 antibody (Cat#R0003, 1:2,000). Green, isotype control; red, H3C1.
Immunocytochemical staining of C2C12 cells with anti-Histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000) . Nuclei were stained blue with DAPI; Histone H3 (acetyl K27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded mouse lung using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse skeletal muscle using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse heart using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:2,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    H3C1
  • Catalog：
    R0015
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated Histone H3 (acetyl K27) Rabbit pAb
Aliases H3C1; H3 Clustered Histone 1; HIST1H3A; H3/A; H3FA; Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3.1; Histone H3/A; H3FC HIST1H3C; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I; Histone; H3/J; Histone H3/K; Histone H3/L; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FL; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ
Background

Gene Name: H3C1

NCBI Gene Entry: 8350

UniProt Entry: P68431

Application Information

Molecular Weight: Predicted, 15 kDa, Observed, 17 kDa

Clonality: Rabbit polyclonal antibody

Clone ID: N/A

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human Histone H3 (acetyl K27)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:1,000-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using anti-histone H3 (acetyl K27) antibody (Cat#R0015). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-histone H3 (acetyl K27) antibody (Cat#R0015). Histone H3 (acetyl K27) expression in wild-type (WT) and H3C1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of H3C1 expression in HT-1080 cells using anti-H3C1 antibody (Cat#R0003, 1:2,000). Green, isotype control; red, H3C1.
  • Immunocytochemical staining of C2C12 cells with anti-Histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000) . Nuclei were stained blue with DAPI; Histone H3 (acetyl K27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse lung using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse liver using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse skeletal muscle using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse heart using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:2,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    H3C1
  • Catalog：
    R0015
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using anti-histone H3 (acetyl K27) antibody (Cat#R0015). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-histone H3 (acetyl K27) antibody (Cat#R0015). Histone H3 (acetyl K27) expression in wild-type (WT) and H3C1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of H3C1 expression in HT-1080 cells using anti-H3C1 antibody (Cat#R0003, 1:2,000). Green, isotype control; red, H3C1.
Immunocytochemical staining of C2C12 cells with anti-Histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000) . Nuclei were stained blue with DAPI; Histone H3 (acetyl K27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded mouse lung using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse skeletal muscle using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse heart using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:2,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-histone H3 (acetyl K27) antibody (Cat#R0015, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  1. CONNECT:[ UseTime:0.000599s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000815s ]
  3. SELECT * FROM `goods` WHERE `code` = 'R0015' LIMIT 1 [ RunTime:0.000518s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000409s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000359s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000382s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 346 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000450s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000474s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 346 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000280s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000447s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000293s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054268s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10302,76,299,5479,7713,9294,8847,9120,7093,6634) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000866s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000454s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000229s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000518s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000275s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000311s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000405s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000259s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000332s ]
0.094160s