Western blotting analysis using growth factor receptor bound protein 7 antibody (Cat#61153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with growth factor receptor bound protein 7 antibody (Cat#61153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using growth factor receptor bound protein 7 antibody (Cat#61153). Growth factor receptor bound protein 7 expression in wild type (WT) and growth factor receptor bound protein 7 (GRB7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with growth factor receptor bound protein 7 antibody (Cat#61153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of growth factor receptor bound protein 7 expression in HepG2 cells using growth factor receptor bound protein 7 antibody (Cat#61153, 1:2,000). Green, isotype control; red, growth factor receptor bound protein 7.