KO-Validated GABARAP Recombinant Rabbit mAb - Product

KO-Validated GABARAP Recombinant Rabbit mAb

#71192

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Recombinant Antibody
Knockout Validated

Western blotting analysis using GABARAP antibody (Cat#71192). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GABARAP antibody (Cat#71192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using GABARAP antibody (Cat#71192). GABARAP expression in wild-type (WT) and GABARAP knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GABARAP antibody (Cat#71192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of GABARAP expression in HepG2 cells using GABARAP antibody (Cat#71192, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, GABARAP.

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Unit price：

Quantity：

(Excluding sales tax)

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Gene Symbol：

GABARAP
Catalog：

71192
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KO-Validated GABARAP Recombinant Rabbit mAb
Aliases	GABARAP; GABA Type A Receptor-Associated Protein; MM46; GABA(A) Receptor-Associated Protein; ATG8A; Gamma-Aminobutyric Acid Receptor-Associated Protein; Epididymis Secretory Sperm Binding Protein; GABARAP-A; FLC3B
Background	Gene Name: GABARAP NCBI Gene Entry: 11337 UniProt Entry: O95166
Application Information	Molecular Weight: Predicted, 14 kDa; observed, 17 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB2405 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human GABARAP
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using GABARAP antibody (Cat#71192). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GABARAP antibody (Cat#71192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using GABARAP antibody (Cat#71192). GABARAP expression in wild-type (WT) and GABARAP knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GABARAP antibody (Cat#71192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of GABARAP expression in HepG2 cells using GABARAP antibody (Cat#71192, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, GABARAP.

Please select the specifications of the product.

Specification：

Unit price：

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

GABARAP
Catalog：

71192
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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