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Home>Product>Primary Antibody

Serpin Family H Member 1 Recombinant Rabbit mAb

#3143

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Recombinant Antibody
Western blotting analysis using Hsp47 antibody (Cat#3143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp47 antibody (Cat#3143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with Hsp47 antibody(Cat#3143, 1:1,000). Nuclei were stained blue with DAPI; Hsp47 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Hsp47 expression in H9c2 cells using Hsp47 antibody (Cat#3143, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Hsp47.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SERPINH1
  • Catalog：
    3143
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R

Information

Data

Information
Product Name Serpin Family H Member 1 Recombinant Rabbit mAb
Aliases SERPINH1; Serpin Family H Member 1; HSP47; SERPINH2; CBP1; CBP2;
Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Serpin Peptidase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Cell Proliferation-Inducing Gene 14 Protein; Rheumatoid Arthritis-Related Antigen RA-A47; Arsenic-Transactivated Protein 3; Collagen Binding Protein 1; 47 KDa Heat Shock Protein; Heat Shock Protein 47; Serpin H1; Colligin; AsTP3; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2, (Collagen-Binding Protein 2); Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2; Rheumatoid Arthritis Antigen A-47; Collagen-Binding Protein; Colligin-1; Colligin-2; RA-A47; PIG14; PPROM; OI10; Gp46
Background

Gene Name: SERPINH1

NCBI Gene Entry: 871

UniProt Entry: P50454

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 46 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5130

Species Reactivity: Mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Hsp47
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Hsp47 antibody (Cat#3143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp47 antibody (Cat#3143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of H9c2 cells with Hsp47 antibody(Cat#3143, 1:1,000). Nuclei were stained blue with DAPI; Hsp47 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Hsp47 expression in H9c2 cells using Hsp47 antibody (Cat#3143, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Hsp47.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SERPINH1
  • Catalog：
    3143
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R
RELATED PRODUCTS
Western blotting analysis using Hsp47 antibody (Cat#3143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp47 antibody (Cat#3143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with Hsp47 antibody(Cat#3143, 1:1,000). Nuclei were stained blue with DAPI; Hsp47 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Hsp47 expression in H9c2 cells using Hsp47 antibody (Cat#3143, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Hsp47.
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